RNA
Part:BBa_K4829023:Design
Designed by: Mrigank Pawagi, Das Labs, Aditya Kamath Ammembal Group: iGEM23_IISc-Bengaluru (2023-10-09)
Ribotree optimised sequence for the CDS of BBa_K4829016
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 145
Illegal BamHI site found at 463
Illegal BamHI site found at 704 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 178
Design Notes
These sequences were optimised by software. The one thing to keep in mind is that it can't be used to optimise UTRs, as they are, very often, not multiples of three, and Ribotree attempts codon optimisation. (However, in UTRs, there are no codons!)
Source
This is not derived from any organism to the best of our knowledge.