RNA

Part:BBa_K4829023:Design

Designed by: Mrigank Pawagi, Das Labs, Aditya Kamath Ammembal   Group: iGEM23_IISc-Bengaluru   (2023-10-09)


Ribotree optimised sequence for the CDS of BBa_K4829016


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 145
    Illegal BamHI site found at 463
    Illegal BamHI site found at 704
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 178


Design Notes

These sequences were optimised by software. The one thing to keep in mind is that it can't be used to optimise UTRs, as they are, very often, not multiples of three, and Ribotree attempts codon optimisation. (However, in UTRs, there are no codons!)




Source

This is not derived from any organism to the best of our knowledge.

References